Part:BBa_K3822004
Hypermethylated region of SDC2
The sequence of Hypermethylated region of SDC2
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Result
According to the advice given by Dr. Xiaoqing, "Multiple genetic testing can improve the accuracy and sensitivity of the product, but too many genes will bring about cost increase, complexity, and other negative effects". We decided to add another gene SDC2,together with TFPI2, as a detector to determine whether it is colorectal cancer.
Same to TFPI2, we conducted a lot of literature research, selected a methylation-enriched region of SDC2 and designed methylation-specific primers. T7 promoter was added to its forward primer and trigger primer was added to its reverse primer. The primer we designed are below:
SDC2-M: methylation specific primer
F: 5′- TAATACGACTCACTATAGGGGTGTAGAAATTAATAAGTGAGAGGG-3′
R: 5′- TATGTAATTGATTTGGCTTCTGTTAGTTTCATATTTCTACTTACAACCAAAACAAAAC-3′
PCR product:264bp (The red is T7 and the blue is Trigger)
Due to time reasons, we did not conduct experimental verification, but the exact same experiment as TFPI2 determines the success of the experiment.
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